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spinning disk confocal superresolution microscope  (Olympus)


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    Olympus spinning disk confocal superresolution microscope
    Spinning Disk Confocal Superresolution Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 24448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spinning+disk+confocal+superresolution+microscope/FV3000+Confocal+Laser+Scanning+Microscope/pm40199910-325-5-10
    Average 99 stars, based on 24448 article reviews
    spinning disk confocal superresolution microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Neuronal DSCAM regulates the peri-synaptic localization of GLAST in Bergmann glia for functional synapse formation.
    Article Snippet: The nanobody fused with Alexa647 (FluoTag-X2 anti-ALFAconjugatedAlexa647,N1502-AF647-L, NanoTag Biotechnologies, Gottingen, Germany) was incubated with secondary antibodies. .. Fluorescence imaging was performed using a Zeiss LSM 780 confocal microscope system (Carl Zeiss, Oberkochen, Germany) and ZEN 2009 software (Carl Zeiss) or spinning disk confocal superresolution microscope (SpinSR10, Olympus Corporation, Tokyo, Japan) and cellSens Dimension (Ver 3.1.1, Olympus Corporation). .. To detect DSCAM-ALFA at both low and high magnifications, a Leica Stellaris 5 laser scanning confocal microscope (Leica, Wetzlar, Germany) was used and images were obtained with the LASX software (Leica).

    Imaging:

    Article Title: Neuronal DSCAM regulates the peri-synaptic localization of GLAST in Bergmann glia for functional synapse formation.
    Article Snippet: The nanobody fused with Alexa647 (FluoTag-X2 anti-ALFAconjugatedAlexa647,N1502-AF647-L, NanoTag Biotechnologies, Gottingen, Germany) was incubated with secondary antibodies. .. Fluorescence imaging was performed using a Zeiss LSM 780 confocal microscope system (Carl Zeiss, Oberkochen, Germany) and ZEN 2009 software (Carl Zeiss) or spinning disk confocal superresolution microscope (SpinSR10, Olympus Corporation, Tokyo, Japan) and cellSens Dimension (Ver 3.1.1, Olympus Corporation). .. To detect DSCAM-ALFA at both low and high magnifications, a Leica Stellaris 5 laser scanning confocal microscope (Leica, Wetzlar, Germany) was used and images were obtained with the LASX software (Leica).

    Microscopy:

    Article Title: Neuronal DSCAM regulates the peri-synaptic localization of GLAST in Bergmann glia for functional synapse formation.
    Article Snippet: The nanobody fused with Alexa647 (FluoTag-X2 anti-ALFAconjugatedAlexa647,N1502-AF647-L, NanoTag Biotechnologies, Gottingen, Germany) was incubated with secondary antibodies. .. Fluorescence imaging was performed using a Zeiss LSM 780 confocal microscope system (Carl Zeiss, Oberkochen, Germany) and ZEN 2009 software (Carl Zeiss) or spinning disk confocal superresolution microscope (SpinSR10, Olympus Corporation, Tokyo, Japan) and cellSens Dimension (Ver 3.1.1, Olympus Corporation). .. To detect DSCAM-ALFA at both low and high magnifications, a Leica Stellaris 5 laser scanning confocal microscope (Leica, Wetzlar, Germany) was used and images were obtained with the LASX software (Leica).

    Article Title: Immunoprofiling of Severity and Stage of Bacterial Infectious Diseases by Ultrabright Fluorescent Nanosphere-Based Dyad Test Strips.
    Article Snippet: Bacterial infectious diseases are common clinical diseases that seriously threaten human health, especially in countries and regions with poor environmental hygiene.. Due to the lack of characteristic clinical symptoms and signs, it is a challenge to distinguish a bacterial infection from other infections, leading to misdiagnosis and antibiotic overuse.. Therefore, there is an urgent need to develop a specific method for detection of bacterial infections.

    Article Title: GPAT4 sustains endoplasmic reticulum homeostasis in endocardial cells and safeguards heart development.
    Article Snippet: .. Cells were viewed with a spinning disk confocal superresolution microscope (Olympus, FV3000) at the following wavelengths: 549 nm (excitation) and 578 nm (emission), and the frame rate was 2 frames/s. .. TMRE: ForMitochondrialmembranepotential assay, treated cells were loadedwith the potentiometric dye 500 nMTMRE (Beyotime, C2001S) at 37 °C for 30min and the staining was viewed by a confocal scanning microscope after washing.

    Software:

    Article Title: Neuronal DSCAM regulates the peri-synaptic localization of GLAST in Bergmann glia for functional synapse formation.
    Article Snippet: The nanobody fused with Alexa647 (FluoTag-X2 anti-ALFAconjugatedAlexa647,N1502-AF647-L, NanoTag Biotechnologies, Gottingen, Germany) was incubated with secondary antibodies. .. Fluorescence imaging was performed using a Zeiss LSM 780 confocal microscope system (Carl Zeiss, Oberkochen, Germany) and ZEN 2009 software (Carl Zeiss) or spinning disk confocal superresolution microscope (SpinSR10, Olympus Corporation, Tokyo, Japan) and cellSens Dimension (Ver 3.1.1, Olympus Corporation). .. To detect DSCAM-ALFA at both low and high magnifications, a Leica Stellaris 5 laser scanning confocal microscope (Leica, Wetzlar, Germany) was used and images were obtained with the LASX software (Leica).

    Incubation:

    Article Title: Immunoprofiling of Severity and Stage of Bacterial Infectious Diseases by Ultrabright Fluorescent Nanosphere-Based Dyad Test Strips.
    Article Snippet: Bacterial infectious diseases are common clinical diseases that seriously threaten human health, especially in countries and regions with poor environmental hygiene.. Due to the lack of characteristic clinical symptoms and signs, it is a challenge to distinguish a bacterial infection from other infections, leading to misdiagnosis and antibiotic overuse.. Therefore, there is an urgent need to develop a specific method for detection of bacterial infections.



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    spinning disk superresolution confocal microscope  (Olympus)
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    Olympus spinning disk superresolution confocal microscope
    HeLa cells in prophase stably expressing mCherry-H2B and transiently transfected with GFP-NuMA (1411-2115) [Movie S1], GFP-NuMA (2058-2115) [Movie S2] or GFPNuMA (1411-2057) [Movie S3]. The GFP signal is in green, and the mCherry signal is in red. Time-lapse videos were acquired using a laser-scanning confocal microscope (Olympus FV 3000). Time is in minutes (min). Note that NuMA does not localize at chromatin at prophase in cells expressing GFP-NuMA (1411-2057) .
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    Image Search Results


    HeLa cells in prophase stably expressing mCherry-H2B and transiently transfected with GFP-NuMA (1411-2115) [Movie S1], GFP-NuMA (2058-2115) [Movie S2] or GFPNuMA (1411-2057) [Movie S3]. The GFP signal is in green, and the mCherry signal is in red. Time-lapse videos were acquired using a laser-scanning confocal microscope (Olympus FV 3000). Time is in minutes (min). Note that NuMA does not localize at chromatin at prophase in cells expressing GFP-NuMA (1411-2057) .

    Journal: Molecular Biology of the Cell

    Article Title: NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit

    doi: 10.1091/mbc.E20-06-0415

    Figure Lengend Snippet: HeLa cells in prophase stably expressing mCherry-H2B and transiently transfected with GFP-NuMA (1411-2115) [Movie S1], GFP-NuMA (2058-2115) [Movie S2] or GFPNuMA (1411-2057) [Movie S3]. The GFP signal is in green, and the mCherry signal is in red. Time-lapse videos were acquired using a laser-scanning confocal microscope (Olympus FV 3000). Time is in minutes (min). Note that NuMA does not localize at chromatin at prophase in cells expressing GFP-NuMA (1411-2057) .

    Article Snippet: Higher-resolution imaging was conducted on an Olympus spinning disk superresolution confocal microscope (IXplore SpinSR) using a 100× (1.45 NA) objective.

    Techniques:

    HeLa cells in prophase stably expressing mCherry‐H2B and transiently transfected with GFP‐NuMA (1411‐2115) [Movie S1], GFP‐NuMA (2058‐2115) [Movie S2] or GFPNuMA (1411‐2057) [Movie S3]. The GFP signal is in green, and the mCherry signal is in red. Time‐lapse videos were acquired using a laser‐scanning confocal microscope (Olympus FV 3000). Time is in minutes (min). Note that NuMA does not localize at chromatin at prophase in cells expressing GFP‐NuMA (1411‐2057) .

    Journal: Molecular Biology of the Cell

    Article Title: NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit

    doi: 10.1091/mbc.E20-06-0415

    Figure Lengend Snippet: HeLa cells in prophase stably expressing mCherry‐H2B and transiently transfected with GFP‐NuMA (1411‐2115) [Movie S1], GFP‐NuMA (2058‐2115) [Movie S2] or GFPNuMA (1411‐2057) [Movie S3]. The GFP signal is in green, and the mCherry signal is in red. Time‐lapse videos were acquired using a laser‐scanning confocal microscope (Olympus FV 3000). Time is in minutes (min). Note that NuMA does not localize at chromatin at prophase in cells expressing GFP‐NuMA (1411‐2057) .

    Article Snippet: Higher-resolution imaging was conducted on an Olympus spinning disk superresolution confocal microscope (IXplore SpinSR) using a 100× (1.45 NA) objective.

    Techniques:

    HeLa cells in prophase stably expressing mCherry‐H2B and transiently transfected with GFP‐NuMA (1411‐2115) [Movie S1], GFP‐NuMA (2058‐2115) [Movie S2] or GFPNuMA (1411‐2057) [Movie S3]. The GFP signal is in green, and the mCherry signal is in red. Time‐lapse videos were acquired using a laser‐scanning confocal microscope (Olympus FV 3000). Time is in minutes (min). Note that NuMA does not localize at chromatin at prophase in cells expressing GFP‐NuMA (1411‐2057) .

    Journal: Molecular Biology of the Cell

    Article Title: NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit

    doi: 10.1091/mbc.E20-06-0415

    Figure Lengend Snippet: HeLa cells in prophase stably expressing mCherry‐H2B and transiently transfected with GFP‐NuMA (1411‐2115) [Movie S1], GFP‐NuMA (2058‐2115) [Movie S2] or GFPNuMA (1411‐2057) [Movie S3]. The GFP signal is in green, and the mCherry signal is in red. Time‐lapse videos were acquired using a laser‐scanning confocal microscope (Olympus FV 3000). Time is in minutes (min). Note that NuMA does not localize at chromatin at prophase in cells expressing GFP‐NuMA (1411‐2057) .

    Article Snippet: Higher-resolution imaging was conducted on an Olympus spinning disk superresolution confocal microscope (IXplore SpinSR) using a 100× (1.45 NA) objective.

    Techniques:

    HeLa cells stably expressing mCherry‐H2B and depleted of endogenous NuMA by RNAi using siRNAs sequences targeting 3'UTR of NuMA. These cells are transiently transfected with AcGFP‐NuMA [Movie S4], AcGFP‐NuMA (1‐2057) [Movie S5], or AcGFPNuMA (1‐2115m) [Movie S6]. The GFP signal is shown in green, and the mCherry signal is in red. Time is in minutes (min). Time ‘0’ min being the last frame of metaphase before the onset of chromosomes segregation. Time‐lapse videos were acquired using a laser scanning confocal microscope (Olympus FV 3000).

    Journal: Molecular Biology of the Cell

    Article Title: NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit

    doi: 10.1091/mbc.E20-06-0415

    Figure Lengend Snippet: HeLa cells stably expressing mCherry‐H2B and depleted of endogenous NuMA by RNAi using siRNAs sequences targeting 3'UTR of NuMA. These cells are transiently transfected with AcGFP‐NuMA [Movie S4], AcGFP‐NuMA (1‐2057) [Movie S5], or AcGFPNuMA (1‐2115m) [Movie S6]. The GFP signal is shown in green, and the mCherry signal is in red. Time is in minutes (min). Time ‘0’ min being the last frame of metaphase before the onset of chromosomes segregation. Time‐lapse videos were acquired using a laser scanning confocal microscope (Olympus FV 3000).

    Article Snippet: Higher-resolution imaging was conducted on an Olympus spinning disk superresolution confocal microscope (IXplore SpinSR) using a 100× (1.45 NA) objective.

    Techniques:

    HeLa cells stably expressing mCherry‐H2B and depleted of endogenous NuMA by RNAi using siRNAs sequences targeting 3'UTR of NuMA. These cells are transiently transfected with AcGFP‐NuMA [Movie S4], AcGFP‐NuMA (1‐2057) [Movie S5], or AcGFPNuMA (1‐2115m) [Movie S6]. The GFP signal is shown in green, and the mCherry signal is in red. Time is in minutes (min). Time ‘0’ min being the last frame of metaphase before the onset of chromosomes segregation. Time‐lapse videos were acquired using a laser scanning confocal microscope (Olympus FV 3000).

    Journal: Molecular Biology of the Cell

    Article Title: NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit

    doi: 10.1091/mbc.E20-06-0415

    Figure Lengend Snippet: HeLa cells stably expressing mCherry‐H2B and depleted of endogenous NuMA by RNAi using siRNAs sequences targeting 3'UTR of NuMA. These cells are transiently transfected with AcGFP‐NuMA [Movie S4], AcGFP‐NuMA (1‐2057) [Movie S5], or AcGFPNuMA (1‐2115m) [Movie S6]. The GFP signal is shown in green, and the mCherry signal is in red. Time is in minutes (min). Time ‘0’ min being the last frame of metaphase before the onset of chromosomes segregation. Time‐lapse videos were acquired using a laser scanning confocal microscope (Olympus FV 3000).

    Article Snippet: Higher-resolution imaging was conducted on an Olympus spinning disk superresolution confocal microscope (IXplore SpinSR) using a 100× (1.45 NA) objective.

    Techniques:

    HeLa cells stably expressing mCherry‐H2B and depleted of endogenous NuMA by RNAi using siRNAs sequences targeting 3'UTR of NuMA. These cells are transiently transfected with AcGFP‐NuMA [Movie S4], AcGFP‐NuMA (1‐2057) [Movie S5], or AcGFPNuMA (1‐2115m) [Movie S6]. The GFP signal is shown in green, and the mCherry signal is in red. Time is in minutes (min). Time ‘0’ min being the last frame of metaphase before the onset of chromosomes segregation. Time‐lapse videos were acquired using a laser scanning confocal microscope (Olympus FV 3000).

    Journal: Molecular Biology of the Cell

    Article Title: NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit

    doi: 10.1091/mbc.E20-06-0415

    Figure Lengend Snippet: HeLa cells stably expressing mCherry‐H2B and depleted of endogenous NuMA by RNAi using siRNAs sequences targeting 3'UTR of NuMA. These cells are transiently transfected with AcGFP‐NuMA [Movie S4], AcGFP‐NuMA (1‐2057) [Movie S5], or AcGFPNuMA (1‐2115m) [Movie S6]. The GFP signal is shown in green, and the mCherry signal is in red. Time is in minutes (min). Time ‘0’ min being the last frame of metaphase before the onset of chromosomes segregation. Time‐lapse videos were acquired using a laser scanning confocal microscope (Olympus FV 3000).

    Article Snippet: Higher-resolution imaging was conducted on an Olympus spinning disk superresolution confocal microscope (IXplore SpinSR) using a 100× (1.45 NA) objective.

    Techniques:

    HeLa cells stably expressing mCherry‐H2B and transiently transfected with AcGFPNuMA [Movie S7] or AcGFP‐NuMA (1‐2057) [Movies S8‐S10]. The expression of AcGFP‐NuMA (1‐2057) leads to higher‐order assemblies within the nucleus. These assemblies are categorized into three groups: homogenous to puncta formation [Movie S8], homogenous to the solid fibrillar network [Movie S9], or puncta to the solid fibrillar network [Movie S10]. Time‐lapse videos were acquired using a laserscanning confocal microscope (Olympus FV 3000). Time is in hours (hr).

    Journal: Molecular Biology of the Cell

    Article Title: NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit

    doi: 10.1091/mbc.E20-06-0415

    Figure Lengend Snippet: HeLa cells stably expressing mCherry‐H2B and transiently transfected with AcGFPNuMA [Movie S7] or AcGFP‐NuMA (1‐2057) [Movies S8‐S10]. The expression of AcGFP‐NuMA (1‐2057) leads to higher‐order assemblies within the nucleus. These assemblies are categorized into three groups: homogenous to puncta formation [Movie S8], homogenous to the solid fibrillar network [Movie S9], or puncta to the solid fibrillar network [Movie S10]. Time‐lapse videos were acquired using a laserscanning confocal microscope (Olympus FV 3000). Time is in hours (hr).

    Article Snippet: Higher-resolution imaging was conducted on an Olympus spinning disk superresolution confocal microscope (IXplore SpinSR) using a 100× (1.45 NA) objective.

    Techniques:

    HeLa cells stably expressing mCherry‐H2B and transiently transfected with AcGFPNuMA [Movie S7] or AcGFP‐NuMA (1‐2057) [Movies S8‐S10]. The expression of AcGFP‐NuMA (1‐2057) leads to higher‐order assemblies within the nucleus. These assemblies are categorized into three groups: homogenous to puncta formation [Movie S8], homogenous to the solid fibrillar network [Movie S9], or puncta to the solid fibrillar network [Movie S10]. Time‐lapse videos were acquired using a laserscanning confocal microscope (Olympus FV 3000). Time is in hours (hr).

    Journal: Molecular Biology of the Cell

    Article Title: NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit

    doi: 10.1091/mbc.E20-06-0415

    Figure Lengend Snippet: HeLa cells stably expressing mCherry‐H2B and transiently transfected with AcGFPNuMA [Movie S7] or AcGFP‐NuMA (1‐2057) [Movies S8‐S10]. The expression of AcGFP‐NuMA (1‐2057) leads to higher‐order assemblies within the nucleus. These assemblies are categorized into three groups: homogenous to puncta formation [Movie S8], homogenous to the solid fibrillar network [Movie S9], or puncta to the solid fibrillar network [Movie S10]. Time‐lapse videos were acquired using a laserscanning confocal microscope (Olympus FV 3000). Time is in hours (hr).

    Article Snippet: Higher-resolution imaging was conducted on an Olympus spinning disk superresolution confocal microscope (IXplore SpinSR) using a 100× (1.45 NA) objective.

    Techniques:

    HeLa cells stably expressing mCherry‐H2B and transiently transfected with AcGFPNuMA [Movie S7] or AcGFP‐NuMA (1‐2057) [Movies S8‐S10]. The expression of AcGFP‐NuMA (1‐2057) leads to higher‐order assemblies within the nucleus. These assemblies are categorized into three groups: homogenous to puncta formation [Movie S8], homogenous to the solid fibrillar network [Movie S9], or puncta to the solid fibrillar network [Movie S10]. Time‐lapse videos were acquired using a laserscanning confocal microscope (Olympus FV 3000). Time is in hours (hr).

    Journal: Molecular Biology of the Cell

    Article Title: NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit

    doi: 10.1091/mbc.E20-06-0415

    Figure Lengend Snippet: HeLa cells stably expressing mCherry‐H2B and transiently transfected with AcGFPNuMA [Movie S7] or AcGFP‐NuMA (1‐2057) [Movies S8‐S10]. The expression of AcGFP‐NuMA (1‐2057) leads to higher‐order assemblies within the nucleus. These assemblies are categorized into three groups: homogenous to puncta formation [Movie S8], homogenous to the solid fibrillar network [Movie S9], or puncta to the solid fibrillar network [Movie S10]. Time‐lapse videos were acquired using a laserscanning confocal microscope (Olympus FV 3000). Time is in hours (hr).

    Article Snippet: Higher-resolution imaging was conducted on an Olympus spinning disk superresolution confocal microscope (IXplore SpinSR) using a 100× (1.45 NA) objective.

    Techniques:

    HeLa cells stably expressing mCherry‐H2B and transiently transfected with AcGFPNuMA [Movie S7] or AcGFP‐NuMA (1‐2057) [Movies S8‐S10]. The expression of AcGFP‐NuMA (1‐2057) leads to higher‐order assemblies within the nucleus. These assemblies are categorized into three groups: homogenous to puncta formation [Movie S8], homogenous to the solid fibrillar network [Movie S9], or puncta to the solid fibrillar network [Movie S10]. Time‐lapse videos were acquired using a laserscanning confocal microscope (Olympus FV 3000). Time is in hours (hr).

    Journal: Molecular Biology of the Cell

    Article Title: NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit

    doi: 10.1091/mbc.E20-06-0415

    Figure Lengend Snippet: HeLa cells stably expressing mCherry‐H2B and transiently transfected with AcGFPNuMA [Movie S7] or AcGFP‐NuMA (1‐2057) [Movies S8‐S10]. The expression of AcGFP‐NuMA (1‐2057) leads to higher‐order assemblies within the nucleus. These assemblies are categorized into three groups: homogenous to puncta formation [Movie S8], homogenous to the solid fibrillar network [Movie S9], or puncta to the solid fibrillar network [Movie S10]. Time‐lapse videos were acquired using a laserscanning confocal microscope (Olympus FV 3000). Time is in hours (hr).

    Article Snippet: Higher-resolution imaging was conducted on an Olympus spinning disk superresolution confocal microscope (IXplore SpinSR) using a 100× (1.45 NA) objective.

    Techniques:

    HeLa Kyoto cells stably expressing mCherry‐LaminB1 and transiently transfected with AcGFP‐NuMA (1‐2057) . The individual cells is imaged in such a way that the entire nucleus is covered with optical sectioning of 0.3 μm. Images were acquired using a laser‐scanning confocal microscope (Olympus FV 3000) and the entire z‐stack (61 images, covering a thickness of 20 μm) was utilized to make 3D‐movie on Imaris (Bitplane Inc.).

    Journal: Molecular Biology of the Cell

    Article Title: NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit

    doi: 10.1091/mbc.E20-06-0415

    Figure Lengend Snippet: HeLa Kyoto cells stably expressing mCherry‐LaminB1 and transiently transfected with AcGFP‐NuMA (1‐2057) . The individual cells is imaged in such a way that the entire nucleus is covered with optical sectioning of 0.3 μm. Images were acquired using a laser‐scanning confocal microscope (Olympus FV 3000) and the entire z‐stack (61 images, covering a thickness of 20 μm) was utilized to make 3D‐movie on Imaris (Bitplane Inc.).

    Article Snippet: Higher-resolution imaging was conducted on an Olympus spinning disk superresolution confocal microscope (IXplore SpinSR) using a 100× (1.45 NA) objective.

    Techniques: